How long to refrain from enemas and/or ozone insufflation before collecting a stool sample?

Please allow at least two weeks to be safe, but we don't really know. Those therapies will definitely impact the large intestine microbiome but there is no solid research on recovery time. The research suggests for colonoscopy prep (which will impact the large intestine microbiome a lot more than enemas), the microbiome will recover in 2-4 weeks. An enema and ozone insufflation impact a much smaller portion of the large intestine microbiome but the portion that is most well represented in a stool sample.