Analysis Questions
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How can I get an RDP analysis done for my sample?
Firstly, RDP stands for Ribosomal Database Project. While we use a curated and more recently updated version of Greengenes in the BIomesight UI, you can request the RDP analysis by following these steps: Toggle to a family account if you haven't already - this will give you access to a Dashboard... -
How can I see how Biomesight calculates the percentages for Butyrate, toxins, etc?
1. Go to the section you are interested in understanding further, in this case we will look at Butyrate: https://biomesight.com/metabolites?category=scfa 2. Expand the Butyrate section by clicking on the section or pressing the + button to expand. 3. Select the "List" tab to see the full list of ... -
Is it possible to see how common zero values are?
You can get an idea of that by looking at the distribution for the bacteria in question. The distribution can be accessed either by: 1. Clicking on the distribution tab (for the bacteria we focus on) or 2. On the boxplot chart available under the breakdown or advanced sections In the chart a... -
What does a zero result for a bacteria mean? Does it mean the bacteria is extinct?
Tests such as Biomesight do not have the sensitivity of targeted qPCR tests. We can only detect a bacteria if it's present at a relative abundance of at least 0.001% of your microbiome. Many species can be present and can even cause symptoms at levels below this. Essentially, that means that if y... -
What is the difference between Probiotics and Commensals
The bacteria under the Probiotics category in Biomesight are also commensals in the human gut. We've split them into 2 categories as follows: Probiotics - Known probiotics that's also available as supplements (E.g. Akkermansia, Lactobacillus and Bifidobacteria) and butyrate producers (Faecalibact... -
When viewing the bacteria breakdown, why are some columns empty?
The sequencing technology combined with the bioinformatics analysis pipeline we use is not always able to determine the full classification of a bacteria down to species level. In this case, it will classify as specific as possible, for example in the screenshot below, we have bacteria from the B... -
The calculations on all the pop-up screens filter out the outliers (the top and bottom 2%) to allow for a more accurate calculation while the values within the table are based on all the values without filters. The mean values in particular can be affected more. This decision was made for perform...